Chromatin immunoprecipitation (ChIP) is a powerful technique for studying the association of certain proteins with specific regions of the genome. These sequence specific DNA-binding proteins are believed to play a role in such cellular processes as DNA replication, transcription regulation, recombination, repair, and segregation; chromosomal stability; cell-cycle progression; and epigenetic silencing.

Chromatin immunoprecipitation sequencing (ChIP-seq) is the evolution of an earlier microarray method (ChIP-to-chip) to enrich and map binding sites for transcription factors, chromatin-modifying complexes, histone modifications, and other chromatin-associated proteins. ChIP-seq is one of the most technically complicated Next Generation Sequencing projects to undertake, and is entirely dependent upon the quality of sample (success of immunoprecipitation) submitted for library preparation.

Service is available on both platforms.

Sample specifications: Since most ChIPed DNA is not measurable by Bioanalyzer or Qubit, qPCR data on a few positive sites would be ideal before proceeding to the library amplification protocol.