Ion Torrent Ion S5™ System

Ion Torrent pairs semiconductor technology with a basic, well-characterized biochemical process to perform massively parallel sequencing without using the light-releasing reactions and sensitive cameras required for other sequencing platforms. Ion Torrent™ Technology directly translates chemically encoded information (A, C, G,T) into digital information (0, 1) on a semiconductor chip. This provides a direct link between chemical and digital data, allowing for a fast, scalable sequencing solution at an affordable price.
The Ion S5™ next-generation sequencing system enables a simple targeted sequencing workflow at an affordable price, without compromising on performance or reliability. It leverages the speed of semiconductor sequencing with impressive on-board computing power, to enable the production of high quality sequencing data in as little as 2.5 hours and enables to go from DNA library to data in as little as 24 hours. The Ion S5 System is simple to use with cartridge-based reagents and offers superior scalability and flexibility to support a broad range of high throughput sequencing applications.

Applications:
• Small Genome Sequencing- Microbial and viral de novo & resequencing
• Targeted Resequencing
• Protein-DNA/RNA interactions (ChIP-Seq)
• Amplicon Sequencing
• Cancer Gene Panel / Custom designed Gene Panel
• Inherited Disease Panel
• Whole Genome/Exome Validation (validate other platform results)
• Copy Number Analysis, Methylation, Paired Ends

Expected Results:
• Ion 520TM chip: 4-6 million reads ,0.6-1Gb of 200bp reads or 1.2-2 Gb of 400bp reads
• Ion 530TM chip: 15-20 million reads ,3-4Gb of 200bp reads or 6-8Gb of 400bp reads
• Ion 540TM chip: 60-80 million reads ,10-15Gb of 200bp reads

Illumina NextSeq500 Sequencer

The NextSeq 500 System uses the same proven reversible-terminator sequencing by synthesis (SBS) technology used by all Illumina NGS systems, with recent innovations reducing cycle and data processing times. SBS technology is the most successful and widely adopted NGS technology worldwide. This technology is able to produce gigabases of high quality sequence information in a single run.

Applications:
• All exome sequencing
• Protein-DNA/RNA interactions (ChIP-Seq)
• RNA sequencing (Full-seq and 3’quant-seq)
• Targeted resequencing

Expected Results:
130-400 M number of reads and 50-100 Gb, depending on experimental needs