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Protein Purification Facility

Protein Purification Facility
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WYATT DAWN 8+

Advanced 8 angles Multi-Angle static Light Scattering (MALS) detector with integrated Wyatt QELS Dynamic Light Scattering (DLS) module.

  • MW Range: from 200 Da to 100 MDa
  • Molar Mass Range: < 10³ - 107 g/mol
  • Sensitivity: 0.5 μg/ml BSA (66.4 kDa) in PBS, 25ngr/ml 100kDa polystyrene in THF
  • Molecular size range (rg): from 10 to 300 nm. Up to 600 nm with shape-specific models
  • Laser wavelength: 685 nm (50mW GaAs linear polarized laser)
  • All solvents compatible (aqueous and organic)

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WYATT DAWN EOS

Advanced 18 angles Multi-Angle static Light Scattering (MALS) detector

  • MW Range: from 200 Da to 100 MDa
  • Molar Mass Range: < 10³ - 107 g/mol
  • Sensitivity: 0.5 μg/ml BSA (66.4 kDa) in PBS, 25ngr/ml 100kDa polystyrene in THF
  • Molecular size range (rg): from 10 to 300 nm. Up to 600 nm with shape-specific models
  • Laser wavelength: 685 nm (50mW GaAs linear polarized laser)
  • All solvents compatible (aqueous and organic)

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WYATT QELS (Quasi-Elastic Light Scattering) Module

The WYATT QELS (Quasi-Elastic Light Scattering) Module on line with the Wyatt DAWN EOS MALS detector adds Dynamic Light Scattering (DLS) capabilities by transferring light collected in the MALS detector via optical fiber to DLS detector.

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COMET (Cell Operation and Maintenance Enhancing Technology)

The COMET (Cell Operation and Maintenance Enhancing Technology) is an ultrasonic transducer that agitates the flow cell and loosens particles adhering to the cell walls windows.

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Size Exclusion Chromatography (SEC) HPLC system 1 with On-line MALS and DLS - Station 1

Size Exclusion Chromatography (SEC) HPLC system 1 with on line Multi-Angle Laser Scattering (MALS) and Quasi Elastic Laser Scattering (QELS) or Dynamic Light Scattering (DLS) is consisted by:

  • A SHIMADZU CBM-20A HPLC system
  • A Wyatt DAWN 8+ advanced 8 angles Multi-Angle static Light Scattering (MALS) detector with integrated Wyatt QELS Dynamic Light Scattering (DLS) module

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Size Exclusion Chromatography (SEC) HPLC system 2 with On-line MALS and DLS - Station 2

Size Exclusion Chromatography (SEC) HPLC system 2 with on line Multi-Angle Laser Scattering (MALS) and Quasi Elastic Laser Scattering (QELS) or Dynamic Light Scattering (DLS) is consisted by:

  • A SHIMADZU SLC 10A VP Complete HPLC system
  • A Wyatt DAWN EOS advanced 18 angles Multi-Angle static Light Scattering (MALS) detector
  • A Wyatt Quasi-Elastic Light Scattering (QELS) or Dynamic Light Scattering (DLS) module