Panagiotis N. Moschou
Collaborating Faculty Member (Associate Professor, University of Crete)
We study basic mechanisms regulating development, cell fate transitions and stress adaptation in dynamic stem cell populations. Our research aims at understanding how stem cells perceive endogenous or exogenous cues and how these are intertwined with structural and mechanical properties of cells.
We mainly focus on homeostasis of proteins and RNA molecules emphasizing on catabolic processes. Our main biological system is the model Arabidopsis. We are experienced in a wide array of experimental platforms of cell biology, biochemistry, genetics and genome editing. Furthermore, we try to extend our basic knowledge in the improvement of Brassicaceae species important for ecology, food and biodiesel (e.g. rapeseed, Camelina).
Ma Y, Guo H, Hu L, Pons Martinez P, Moschou PN, Cevik V, Ding P, Duxbury Z, Sarris PF, Jones JDG. (2018) Distinct modes of derepression of an Arabidopsis immune receptor complex by two different bacterial effectors. PNAS 115:10218-10227.
Minina EA, Reza SH, Gutierrez-Beltran E, Elander PH, Bozhkov PV, Moschou PN. (2017) The Arabidopsis homolog of Scc4/MAU2 is essential for embryogenesis. J. Cell Sci. 130:1051-1063.
Liu C, Moschou PN. (2017) Cutting in the middleman: hidden substrates at the interface between proteases and plant development. New Phytol. 218:916-922.
Moschou PN, Gutierrez-Beltran E, Bozhkov PV, Smertenko A. (2016) Separase Promotes Microtubule Polymerization by Activating CENP-E-Related Kinesin Kin7. Dev. Cell. 37:350-361.
Moschou PN, Savenkov EI, Minina EA, Fukada K, Reza HS, Gutierrez-Beltran E, Sanchez-Vera V, Suarez MF, Hussay PJ, Smertenko AP, Bozhkov PV. (2016) EXTRA SPINDLE POLES (Separase) controls anisotropic cell expansion in Norway spruce (Picea abies) embryos independently from its role in anaphase progression. New Phytol. 212:232-43.
Full publication list
Correspondence indicated by §; only accepted or published papers
2018
Ma Y., Guo H., Hu L., Pons Martinez P., Moschou P.N., Cevik V., Ding P., Duxbury Z., Sarris P.F., Jones J.D.G. 2018 Distinct modes of derepression of an Arabidopsis immune receptor complex by two different bacterial effectors. Proceedings of the National Academy of Sciences USA (in press)
Tornkvist A., Liu C., Moschou P.N. Proteolysis in Nitrogen metabolism: Emerging insights. Journal of Experimental Botany (in press)
§Liu C., Moschou P.N. Cutting in the middleman: hidden substrates at the interface between proteases and plant development. New Phytologist 10.1111/nph.14501
§Liu C., Moschou P.N. Phenotypic novelty by CRISPR in plants. Developmental Biology 10.1016/j.ydbio.2018.01.015
Minina E.A., Moschou P.N., Vetukuri R.R., Sanchez-Vera V., Cardoso C., Liu Q., Elander P.H., Dalman K., Beganovic M., Lindberg Yilmaz J., Marmon S., Shabala L., Suarez M.F., Ljung K., Novák O., Shabala S., Stymne S., Hofius D., Bozhkov P.V. Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness. Journal of Experimental Botany 10.1093/jxb/ery010
§Moschou P.N. Determination of di–/polyamine oxidase activity in plants by an in-gel spermidine oxidation assay. Methods in Molecular Biology 10.1007/978-1-4939-7398-9_14
Dolfors F., Holmquist L., Moschou P.N., Dixelius C., Tzelepis G. The Rhizoctonia solani LysM and RlpA-like effector proteins contribute to virulence suppressing chitin-triggered immunity and hypersensitive response. Biorxiv 10.1101/395582
2017
§Minina E.A., Moschou P.N., Bozhkov P.V. Limited and digestive proteolysis: crosstalk between evolutionary conserved pathways. New Phytologist 10.1111/nph.14627
§Minina E.A., Reza S.H., Gutierrez-Beltran E., Elander P.H., Bozhkov P.V., Moschou P.N. The Arabidopsis homolog of Scc4/MAU2 is essential for embryogenesis. Journal of Cell Science 10.1242/jcs.196865
Minina E.A., Reza S.H., Gutierrez-Beltran E., Elander P.H., Bozhkov P.V., Moschou P.N. Highlight in Development: The Arabidopsis homolog of Scc4/MAU2 is essential for embryogenesis. Development, 144:e1.2-e1.2
§Liu C., Stael S., Gevaert K., Van Breusegem F., Bozhkov P.V., Moschou P.N. The Proteolytic Landscape of an Arabidopsis Separase-Deficient Mutant Reveals Novel Substrates Associated With Plant Development. Biorxiv 10.1101/140962
2016
§Gémes K., Kim Y.J., Park K.Y., Moschou P.N., Andronis E., Valassaki C., Roussis A., Roubelakis-Angelakis K.A. An NADPH-oxidase/polyamine oxidase feedback loop controls oxidative burst under salinity. Plant Physiology 10.1104/pp.16.01118
§Moschou P.N., Savenkov E.I., Minina E.A., Fukada K., Reza S.H., Gutierrez-Beltran E., Sanchez-Vera V., Suarez M.F., Hussey P.J., Smertenko A.P., Bozhkov P.V. EXTRA SPINDLE POLES (Separase) controls anisotropic cell expansion in Norway spruce (Picea abies) embryos independently of its role in anaphase progression. New Phytologist 10.1111/nph.14012
§Moschou P.N., Gutierrez-Beltran E., Bozhkov P.V., Smertenko A. Separase Promotes Microtubule Polymerization by Activating CENP-E-Related Kinesin Kin7. Developmental Cell 10.1016/j.devcel.2016.04.015
Mellidou I., Moschou P.N., Ioannidis N.E., Pankou C., Gėmes K., Valassakis C., Andronis E.A., Beris D., Haralampidis K., Roussis A., Karamanoli A., Matsi T., Kotzabasis K., Constantinidou H.-I., Roubelakis-Angelakis K.A. Silencing S-adenosyl-L-methionine decarboxylase (SAMDC) in Nicotiana tabacum points at a polyamine-dependent trade-off between growth and tolerance responses. Frontiers in Plant Science 10.3389/fpls.2016.00379
Zhu T., Moschou P.N., Alvarez J.M., Sohlberg J.J., Arnold S. WUSCHEL-RELATED HOMEOBOX 2 is important for protoderm and suspensor development in the gymnosperm Norway spruce. BMC Plant Biology 10.1186/s12870-016-0706-7
Smertenko A., Moschou P.N., Zhang L., Fahy D., Bozhkov P. Characterization of cytokinetic mutants using small fluorescent probes. Methods in Molecular Biology 10.1007/978-1-4939-3142-2_15
2015
§Gutierrez-Beltran E., Moschou P.N., Smertenko A.P., Bozhkov P.V. Tudor staphylococcal nuclease links formation of stress granules and processing bodies with mRNA catabolism in Arabidopsis. Plant Cell 10.1105/tpc.114.134494
Gutiérrez-Beltran E., Bozhkov P.V., Moschou P.N. Tudor staphylococcal nuclease plays two antagonistic roles in RNA metabolism under stress. Plant Signaling and Behavior 10.1080/15592324.2015.1071005
Alvarez J.M., Sohlberg J., Engström P., Zhu T., Englund M., Moschou P.N., von Arnold S. The WUSCHEL-RELATED HOMEOBOX 3 gene PaWOX3 regulates lateral organ formation in Norway spruce. New Phytologist 10.1111/nph.13536
2014
Andronis E.A., Moschou P.N., Toumi I., Roubelakis-Angelakis K.A. Peroxisomal polyamine oxidase and NADPH-oxidase cross-talk for ROS homeostasis which affects respiration rate in Arabidopsis thaliana. Frontiers in Plant Science 10.3389/fpls.2014.00132
§Moschou P.N., Roubelakis-Angelakis K.A. Polyamines and programmed cell death. Journal of Experimental Botany 10.1093/jxb/ert373
Zhu T., Moschou P.N., Alvarez J.M., Sohlberg J.J., Von Arnold S. WUSCHEL-RELATED HOMEOBOX 8/9 is important for proper embryo patterning in the gymnosperm Norway spruce. Journal of Experimental Botany 10.1093/jxb/eru371
2013
§Moschou P.N., Smertenko A.P., Minina E.A., Fukada K., Savenkov E.I., Robert S., Hussey P.J., Bozhkov P.V. The caspase-related protease separase (EXTRA SPINDLE POLES) regulates cell polarity and cytokinesis in Arabidopsis. Plant Cell 10.1105/tpc.113.113043
Minina E.A., Sanchez-Vera V., Moschou P.N., Suarez M.F., Sundberg E., Weih M., Bozhkov P.V. Autophagy mediates caloric restriction-induced lifespan extension in Arabidopsis. Aging Cell 10.1111/acel.12048
2012
Moschou P.N., Wu J., Cona A., Tavladoraki P., Angelini R., Roubelakis-Angelakis K.A. The polyamines and their catabolic products are significant players in the turnover of nitrogenous molecules in plants. Journal of Experimental Botany 10.1093/jxb/ers202
Moschou P.N., Bozhkov P.V. Separases: Biochemistry and function. Physiologia Plantarum 10.1111/j.1399-3054.2011.01550.x
*Fincato P., *Moschou P.N., *Ahou A., Angelini R., Roubelakis-Angelakis K.A., Federico R., Tavladoraki P. The members of Arabidopsis thaliana PAO gene family exhibit distinct tissue- and organ-specific expression pattern during seedling growth and flower development. Amino Acids 10.1007/s00726-011-0999-7 (*equal contributors)
2011
Tisi A., Federico R., Moreno S., Lucretti S., Moschou P.N., Roubelakis-Angelakis K.A., Angelini R., Cona A. Perturbation of polyamine catabolism can strongly affect root development and xylem differentiation. Plant Physiology 10.1104/pp.111.173153
§Moschou P.N., Roubelakis-Angelakis K.A. Characterization, assay, and substrate specificity of plant polyamine oxidases. Methods in molecular biology (Clifton, N.J.) 10.1007/978-1-61779-034-8_11
Fincato P., Moschou P.N., Spedaletti V., Tavazza R., Angelini R., Federico R., Roubelakis-Angelakis K.A., Tavladoraki P. Functional diversity inside the Arabidopsis polyamine oxidase gene family. Journal of Experimental Botany 10.1093/jxb/erq341
2010
Paschalidis K.A., Toumi I., Moschou P.N., Roubelakis-Angelakis K.A. ABA-dependent amine oxidases-derived H2O2 affects stomata conductance. Plant Signaling and Behavior 10.4161/psb.5.9.12679
Wu J., Shang Z., Wu J., Jiang X., Moschou P.N., Sun W., Roubelakis-Angelakis K.A., Zhang S. Spermidine oxidase-derived H2O2 regulates pollen plasma membrane hyperpolarization-activated Ca2+-permeable channels and pollen tube growth. Plant Journal 10.1111/j.1365-313X.2010.04301.x
§Toumi I., Moschou P.N., Paschalidis K.A., Bouamama B., Ben Salem-fnayou A., Ghorbel A.W., Mliki A., Roubelakis-Angelakis K.A. Abscisic acid signals reorientation of polyamine metabolism to orchestrate stress responses via the polyamine exodus pathway in grapevine. Journal of Plant Physiology 10.1016/j.jplph.2009.10.022
§Moschou P.N., Roubelakis-Angelakis K.A. Polyamines in grapevine research. Methodologies and Results in Grapevine Research 10.1007/978-90-481-9283-0_13
2009
Paschalidis K.A., Moschou P.N., Aziz A., Toumi I., Roubelakis-Angelakis K.A. Polyamines in grapevine: An update. Grapevine Molecular Physiology and Biotechnology: Second Edition 10.1007/978-90-481-2305-6_8
Loulakakis K.A., Morot-Gaudry J.F., Velanis C.N., Skopelitis D.S., Moschou P.N., Hirel B., Roubelakis-Angelakis K.A. Advancements in nitrogen metabolism in grapevine Grapevine Molecular Physiology and Biotechnology: Second Edition 10.1007/978-90-481-2305-6_7
Labboun S., Tercé-Laforgue T., Roscher A., Bedu M., Restivo F.M., Velanis C.N., Skopelitis D.S., Moschou P.N., Roubelakis-Angelakis K.A., Suzuki A., Hirel B. Resolving the role of plant glutamate dehydrogenase. I. In vivo real time nuclear magnetic resonance spectroscopy experiments. Plant and Cell Physiology 10.1093/pcp/pcp155
Paschalidis K.A., Moschou P.N., Toumi I., Roubelakis-Angelakis K.A. Polyamine anabolic/catabolic regulation along the woody grapevine plant axis. Journal of Plant Physiology 10.1016/j.jplph.2009.03.013
Moschou P.N., Sarris P.F., Skandalis N., Andriopoulou A.H., Paschalidis K.A., Panopoulos N.J., Roubelakis-Angelakis K.A. Engineered polyamine catabolism preinduces tolerance of tobacco to bacteria and oomycetes. Plant Physiology 10.1104/pp.108.134932
2008
Moschou P.N., Sanmartin M., Andriopoulou A.H., Rojo E., Sanchez-Serrano J.J., Roubelakis-Angelakis K.A. Bridging the gap between plant and mammalian polyamine catabolism: A novel peroxisomal polyamine oxidase responsible for a full back-conversion pathway in arabidopsis. Plant Physiology 10.1104/pp.108.123802
Moschou P.N., Delis I.D., Paschalidis K.A., Roubelakis-Angelakis K.A. Transgenic tobacco plants overexpressing polyamine oxidase are not able to cope with oxidative burst generated by abiotic factors. Physiologia Plantarum 10.1111/j.1399-3054.2008.01049.x
Moschou P.N., Paschalidis K.A., Delis I.D., Andriopoulou A.H., Lagiotis G.D., Yakoumakis D.I., Roubelakis-Angelakis K.A. Spermidine exodus and oxidation in the apoplast induced by abiotic stress is responsible for H2O2 signatures that direct tolerance responses in tobacco. Plant Cell 10.1105/tpc.108.059733
Toumi I., Gargouri M., Nouairi I., Moschou P.N., Salem-Fnayou A.B., Mliki A., Zarrouk M., Ghorbel A. Water stress induced changes in the leaf lipid composition of four grapevine genotypes with different drought tolerance. Biologia Plantarum 10.1007/s10535-008-0035-2
Moschou P.N., Paschalidis K.A., Roubelakis-Angelakis K.A. Plant polyamine catabolism: The state of the art. Plant Signaling and Behavior. 10.4161/psb.3.12.7172
MISCELLANEOUS
…Moschou P.N., +83 authors. 2018. Regulating genome edited organisms as GMOs has negative consequences for agriculture, society and economy. Position paper. VIB, Belgium
K. A. Roubelakis-Angelakis KA, Moschou P.N., Sarris P.F., Panopoulos N.J. 2009. ALTERED POLYAMINE CATABOLISM: PRODUCING TRANSGENIC PLANTS WITH ENHANCED TOLERANCE TO BACTERIA AND OOMYCETES. INFORMATION SYSTEMS FOR BIOTECHNOLOGY.
PATENTS
P41503887SE00. Bozhkov P., Minina A., Stymne S., Moschou P.N., Hofius D; “Improving plant fitness through autophagy”.