MoFlo Cell - SORTER
The facility is equipped with one research-grade Cell-Sorting unit consisting of the DakoCytomation (now Beckman-Coulter) MoFlo High-Performance Cell Sorter with the Summit software program (http://www.coulterflow.com/bciflow/instrumentsot.php).
MOFLO optical layout
MoFlo - Sorter utilizes three Lasers: 1) UV- 355nm, 2) Blue - 488nm, and 3) Red Diode - 643nm
which can measure up to 9 cellular markers according to Fig.1. Several common fluorochromes (FITC, R-PE, PerCp-Cy5.5, PE-Cy7, APC, APC - Cy5.5) and fluorescent GFP protein have been successfully used.
The unique configuration of the Lasers allows simultaneous separation of 4 different populations with a purity greater than 99% and high recovery.
FACSCalibur
FACSCalibur is a bench-top cell analyzer, configured with two lasers:
1) 488 nm Blue-green Argon Ion laser
2) 633 nm Red Helium Neon laser
The FlowJo software is installed on a computer near the FACSCalibur to analyze flow cytometry data.
Three fluorescence channels and two scatter channels are available from the 488 nm laser (FSC, SSC, FL1, FL2, FL3). One fluorescence channel is available from the 633 nm laser (FL4)
