The Fly/Insect facility supports research on Drosophila and other insect species, including species of medical importance (mosquito) and agricultural pests (medfly, olive-fly, flour beetle). It is a multi-user facility for insect genetics and transgenesis, equipped with a state-of-the-art system for embryo injection. In addition to routine transgenesis of Drosophila melanogaster, the facility has produced transgenic lines of Ceratitis caritata (medfly), Bactrocera oleae (olive fly) and Tribolium castaneum (flour beetle). Furthermore, cytoplasmic transfers between different dipteran species for the propagation of bacterial symbionts (Wolbachia) have been successfully carried out.

The facility is built around the central Flyroom, a large lab with four work stations equipped with CO2, anaesthetization stages, stereoscopes and cool light sources. The Flyroom is surrounded by three walk-in environmental chambers with controlled temperature, humidity and light/ dark cycle. An isolated two-room space is dedicated to rearing Anopheles gambiae mosquitoes. Within the mosquito insectarium, infections with the rodent malaria parasite Plasmodium berghei are performed. Dark chambers for fluorescence microscopy complete the setup.

Satellite facilities are the microinjection lab and a fly-food preparation kitchen.